Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against Helicobacter pylori

Figure 2

Evaluation of the H. pylori biofilm formation in the presence of OxOral®. The H. pylori biofilm formation was carried using sterile 22 × 22 mm glass coverslips that were placed in Petri dishes. Each plate was filled with MH broth supplemented and OxOral^® at different doses. Biofilm formation was started by inoculating H. pylori at an initial concentration of 1 × 10⁶ CFU/mL. The dishes were incubated under microaerobic conditions at 37°C for 168 h. After incubation, coverslips were washed and stained with 0.1% violet crystal. After staining, the dye associated to biofilms was dissolved using 33% glacial acetic acid, then the optical density was measured at 600 nm and the percentage of biofilm inhibition was calculated. The data represent the percentage mean ± the percentage deviation. TET, 5 μg/mL tetracycline; SS, saline solution; ∗∗, ; ns, not significant.