Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways

<p>Effect of siNrf2 and PI3K inhibitor on carnosine effect against HG-induced ROS production and cell apoptosis. (a-b) Western blots were performed to detect siNrf2 expression in MPC5 cells (n=3). (c) Mitochondrial ROS were detected by the confocal microscope (n=3). Intracellular ROS were detected by a fluorescent microscope (n=3). Apoptosis of MPC5 cells was detected by TUNEL assay (n=3). (d-e) The protein expression levels of Bax.Bcl-2 and Cleaved caspase-3 were detected by Western blot (n=3); NC: siRNA negative control; HG+CA: high glucose (30mM) plus carnosine (20mM); HG+CA+siNrf2: high glucose (30mM) plus carnosine (20mM) plus siNrf2 (20<i>μ</i>M); HG+CA+LY: high glucose (30mM) plus carnosine (20mM) plus LY294002 (20<i>μ</i>M); data are presented as mean ± SD. <span class="nowrap"><svg height="13.9365pt" id="M18" style="vertical-align:-3.834pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 13.9365" width="50.9184pt" xmlns="http://www.w3.org/2000/svg"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 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BioMed Research International

fig5

Figure 5

Figure 5: Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways 