Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Table 2

Oligonucleotide primer sequences used for detection of ESBL genes and PCR cycling conditions used.

Primer	Primer sequence (5′–3′)	Target gene	Amplicons size (bp)	PCR conditions and cycles	Reference
blaTEMF	AAACGCTGGTGAAAGTA	blaTEM	822	1 cycle of 5 minutes at 94°C, 35 cycles of 30 seconds at 94°C, 1 minute at 45°C, 1 minute at 72°C; 1 cycle of 10 minutes at 72°C	[27]
blaTEMR	AGCGATCTGTCTAT
blaSHVF	ATGCGTTATATTCGCCTGTG	blaSHV	753
blaSHVR	TGCTTTGTTATTCGGGCCAA
blaCTX-MF	CGCTTTGCGATGTGCAG	blaCTX-M	550
blaCTX-MR	ACCGCGATATCGTTGGT
blaOXAF	ATATCTCTACTGTTGCATCTCC	blaOXA	619
blaOXAR	AAACCCTTCAAACCATCC
blaCMY-1F	GTGGTGGATGCCAGCATCC	blaCMY-1	915	1 cycle of 3 minutes at 94°C, 25 cycles of 1 minute at 94°C, 1 minute at 58°C, 1 minute at 72°C; 1 cycle of 10 minutes at 72°C	[26]
blaCMY-1R	GGTCGAGCCGGTCTTGTTGAA
blaCMY-2F	GCACTTAGCCACCTATACGGCAG	blaCMY-2	758
blaCMY-2R	GCTTTTCAAGAATGCGCCAGG