Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa
Table 1
Oligonucleotide primer sequences used for amplification of 16S rRNA universal, uspA, uidA, and ntrA genes and PCR cycling conditions used.
Primers
Sequence (5′–3′)
Targeted gene
Amplicon size (bp)
PCR conditions and cycles
Reference
27F
AGAGTTTGATCATGGCTCAG
16S rRNA
1420
1 cycle of 3 minutes at 94°C, 25 cycles of 1 minute at 94°C, 1 minute at 55°C, 2 minutes at 72°C; 1 cycle of 10 minutes at 72°C