Human Embryonic Stem Cell-Derived Neural Lineages as In Vitro Models for Screening the Neuroprotective Properties of Lignosus rhinocerus (Cooke) Ryvarden
Figure 2
Effects of L. rhinocerus (a) HA and (b) ME extracts (1 - 100 μg/ml) on neurite outgrowth of NSCs after 72 h of incubation. Results are expressed as mean ± SEM (n = 4). Asterisks denote significant differences compared to 0 μg/ml (negative control); P < 0.05, P < 0.01. (c) Images of NSCs treated with 0 μg/ml, B-27 supplement, L. rhinocerus HA extract (10 and 100 μg/ml) and ME extract (5 and 100 μg/ml). (d) Immunostaining and fluorescence microscopy of TUJ-1 expression in NSCs treated with 0 μg/ml, B-27 supplement, L. rhinocerus HA extract (10 μg/ml) and ME extract (5 μg/ml). Nuclei were stained with DAPI (blue) and neurites were stained with TUJ-1 (green). Arrows indicate extension of neurites that was at least twice the length of the cell body diameter. Scale bar: 100 μm.