The Effect of Carbogen Breathing on ¹⁸F-FDG Uptake in Non-Small-Cell Lung Cancer

Figure 4

Effect of carbogen breathing on ¹⁸F-FDG uptake in regions with GLUT-1 overexpression. (a) Under air breathing condition, higher ¹⁸F-FDG accumulation, pimonidazole binding, and GLUT-1 are colocalized. (b) Under carbogen breathing, decrease in ¹⁸F-FDG accumulation and lower pimonidazole binding are found in GLUT-1 overexpressing regions, and Hoechest 33342 binding is not affected indicating no blood perfusion change. H&E: hematoxylin and eosin; GLUT-1-PIMO-Hoechst: overlay of GLUT-1 (red), pimonidazole (green), and Hoechst 33342 (blue); DAR: ¹⁸F-FDG digital autoradiography; scale bars = 2 mm.

(a)

(b)