Downregulating Serine Hydroxymethyltransferase 2 Deteriorates Hepatic Ischemia-Reperfusion Injury through ROS/JNK/P53 Signaling in Mice

Figure 4

Knockdown of SHMT2 aggravates IR-induced apoptosis of hepatocytes. Intracellular generation of ROS in primary hepatocytes by transforming H2DCFDA to DCF via an oxidative reaction and quantification data for the ROS level of each group after hypoxia reoxygenation (scale bars: 50 μm, n ≥ 4, ). (a) NC and scramble were used as controls. Values represent the mean ± SD of at least three independent experiments. (b) The expression of SHMT2, HIF-1α, p-JNK, JNK, p-p53, and p53 measured by western blot analysis after hypoxia reoxygenation. NC and scramble were used as controls. (c) The effect of SHMT2 on hypoxia-induced apoptosis in primary hepatocytes was determined using Annexin V/PI staining and flow cytometry. NC and scramble were used as controls. (d) Quantification of early apoptotic cells. Values represent the mean ± SD of at least three independent experiments.