Downregulating Serine Hydroxymethyltransferase 2 Deteriorates Hepatic Ischemia-Reperfusion Injury through ROS/JNK/P53 Signaling in Mice

Figure 3

Knockdown of SHMT2 aggravates IR-induced apoptosis of hepatocytes. (a) TUNEL staining shows cellular apoptosis in mouse livers from NS, scramble, and shSHMT2 groups at 24 h after IR (original magnification: 400x). (b) Quantification of TUNEL-positive cells in NS, scramble, and shSHMT2 groups at 24 h after IR (; n ≥ 4). (c) Protein abundances of SHMT2, JNK, p-JNK, P53, p-P53, TNF-α, and IL-1 in IR liver tissue from NS, scramble, and shSHMT2 groups at 24 h after IR. Sham was used as a control. Values represent the mean ± SD of at least three independent experiments.