Downregulating Serine Hydroxymethyltransferase 2 Deteriorates Hepatic Ischemia-Reperfusion Injury through ROS/JNK/P53 Signaling in Mice

Figure 2

Knockdown of SHMT2 worsens hepatic IR injury in mice. (a) H&E-stained images show necrotic areas, histological damage, and liver architecture in mouse livers from NS, scramble, and shSHMT2 groups at 24 h after IR (original magnification: 400x). (b) Suzuki scores at 24 h after IR. (c) Total and cleaved protein abundances of caspase-3 in IR liver tissue from NS, scramble, and shSHMT2 groups at 24 h after IR. Serum concentrations of ALT (d) and AST (e) at 4 h, 8 h, 16 h, and 24 h after IR (n ≥ 4, , ). Sham was used as a control. Values represent the mean ± SD of at least three independent experiments.