Tankyrase Promotes Aerobic Glycolysis and Proliferation of Ovarian Cancer through Activation of Wnt/β-Catenin Signaling

Figure 2

The Role of TNKS in the Proliferation of Ovarian Cancer Cells. (a) Western blotting analysis of TNKS protein levels in several ovarian cancer cell lines and normal human ovarian epithelium (NHOE) cells, and the effect of TNKS knockdown on the proliferation of OVCAR-3 cells detected by MTS in shTNKS-OVCAR-3 cells and its control (shCtrl) cells. (n = 3). (b) Effect of XAV939 on the proliferation of different ovarian cell lines detected by MTS (n = 3). There are statistical differences between different time groups or dose groups in ES-2, OVCAR-3, and A2780 cells but not in NHOE cells. (c) Colony formation assays were used to assess the oncogenic potential of TNKS. The relative number of colonies was quantified (n = 3). (d) Tumor growth curve (left) and final weight of shTNKS and shCtrl xenografts in nude mice (n = 6). The shTNKS and shCtrl cells were injected into the flanks of nude mice, tumor diameters measured twice weekly. Data were expressed as the mean ± SD. , P < 0.05; , P < 0.01; , P < 0.01.

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