Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature

Table 4

Results of cloning and sequencing.

Gene	Cloning and sequencing results	Sequence length
TUB	GATTACCACCTGTTACTTGCCCCGAGGATGATCCGTGAACT	116
	CATCCCTGAAGCTGTCCTCGGCTTGTTCGTGCACACACCAT
	TCCCAAGCAGCGAAGTTTTCCGATGCCTACCTCGCCGCAAG
	GAGATACTCGATGGCATGCTAGGCGCCAACCTTGTTTGCTT
	CCAAACATACTCCTACGCTCGTC
MP	CCATCCCCAAAGTCCAAAGAGCTGCAGTTGTGTCCTCAGCT	133
	GGAGCTCCTATCGAAATTCGCCACGATGTACCAGTCAAGCA
	AGCGTCCGAGCTCGCCGCTGGGGAGTGTCTCGTAAAGCTAT
	TCTGCACAGG
MD	TCATCAACCAGGCAGGACTCAACCGCCCTCTCACTCAGCAA	86
	CCGCTGACAGTGTTCTACAACTCGTCCAAGGCTGCGGTGAA
	TAAC
MG	TCCCGAACCAGCGAGAAGACTAGGGATTCATCTACGAAAC	140
	GCACTAGCTTGGTAGCCGCAGTCTGGTCCGTCCTACCTGAT
	GCTTTTACTTCAACAGGTTCCCCCAAGAATTCACGCCTTCGT
	TCCTTATCCGCTTCTTC
MB	CGCCGAAACGCTTGATGGCCGCCTATTGTTCGCGATCCCTA	156
	AAAAAGGGAGATTGCATGAAAAGTGCCTGTCGCTGCTCGC
	AGGCGCCGATATACAGTTCCGAAGGCACAACCGCTTAGAT
	GTGGCCCTTGTATTAAACCACCCTATCGCACTCGT