Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature

Table 3

Primers for RT-qPCR.

Primer	Base number	Gene sequence
MP-F	20	5′-CCATCCCCAAAGTCCAAAGA-3′
MP-R	24	5′-CCTGTGCAGAATAGCTTTACGAGA-3′
MD-F	22	5′-TCATCAACCAGGCAGGACTCAA-3′
MD-R	22	5′-GTTATTCACCGCAGCCTTGGAC-3′
MG-F	17	5′-TCCCGAACCAGCGAGAA-3′
MG-R	22	5′-GAAGAAGCGGATAAGGAACGAA-3′
MB-F	17	5′-CGCCGAAACGCTTGATG-3′
MB-R	20	5′-ACGAGTGCGATAGGGTGGTT-3′