Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature

Table 1

RT-qPCR reaction system.

Reagent	Volume
SYBR Premix Ex TaqTM (2×)	10 μL
Forward primer (10 μmo1 L^−1)	0.4 μL
Reverse primer (10 μmo1 L^−1)	0.4 μL
Rox	0.4 μL
ddH2O	6.8 μL
cDNA	2 μL
Total	20 μL