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BioMed Research International
/
2019
/
Article
/
Tab 1
/
Research Article
Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on
Volvariella volvacea
at Low Temperature
Table 1
RT-qPCR reaction system.
Reagent
Volume
SYBR Premix Ex TaqTM (2×)
10
μ
L
Forward primer (10
μ
mo1 L
−1
)
0.4
μ
L
Reverse primer (10
μ
mo1 L
−1
)
0.4
μ
L
Rox
0.4
μ
L
ddH2O
6.8
μ
L
cDNA
2
μ
L
Total
20
μ
L