Targeting N-Terminal Huntingtin with a Dual-sgRNA Strategy by CRISPR/Cas9

Table 1

Sequences of sgRNAs and PCR primers.

	Sequences (5′-3′)
sgRNA sequences
sgHTT-1	GAGTCGGCCCGAGGCCTCCG
sgHTT-2	GCCTCCGGGGACTGCCGTGCC
sgHTT-3	GCGGGGACTGCCGTGCCGGGC
sgHTT-4	GTGCCGGGCGGGAGACCGCCA
sgHTT-5	GTGAGGAAGCTGAGGAGGCGG
sgHTT-6	GGCTGAGGAAGCTGAGGAGG
sgHTT-7	GGCGGCGGCTGAGGAAGCTG
sgHTT-8	GAGCAGCGGCTGTGCCTGCGG
sgHTT-9	GAGCGGCTCCTCAGCCACAGC
T7E1 assay and junction PCR
HTT-T7E1-F	CCAGCCATTGGCAGAGTCCG
HTT-T7E1-R	TTGCTGGGTCACTCTGTCTCTG
Quantitative PCR
HTT-qF	TGACGCAGAGTCAGATGTCAG
HTT-qR	CCGAGGGGCACCATTCTTTT
GAPDH-qF	CGGGATTGTCTGCCCTAATTAT
GAPDH-qR	GCACGGAAGGTCACGATGT
Sequencing of junctions
HTT-5′UTR/e1-F	CGGGAGACCGGTGGCTGAGG
HTT-e4/e5-R	CCGAGGGGCACCATTCTTTT