UCHL1/PGP 9.5 Dynamic in Neuro-Immune-Cutaneous Milieu: Focusing on Axonal Nerve Terminals and Epidermal Keratinocytes in Psoriatic Itch

Figure 4

Expression of PGP 9.5 by epidermal keratinocytes in control skin and nonlesional and lesional skin with (Itch+) and without (Itch-) itch. Immunofluorescence images of epidermal expression of PGP 9.5 in control healthy skin was found mostly in basal keratinocytes (BKs) with weak detection by suprabasal keratinocytes (SBKs) (a). Further images represent BKs and SBKs PGP 9.5 expression in nonlesional and lesional samples with ((b), (c); Itch+) and without ((d), (e); Itch-) itch. Inserts: western-blot analysis of PGP 9.5 expression of the representative skin samples of two controls as well as two patients with (Itch+) and two without (Itch-) itch, respectively. Representative bands of PGP 9.5 (27kDa) and β-actin (42kDa) endogenous control are listed in the lower right corners of merged images.

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