Decreased Endometrial IL-10 Impairs Endometrial Receptivity by Downregulating HOXA10 Expression in Women with Adenomyosis

Figure 2

IL-10 increases HOXA10 expression via phosphorylation of STAT3. The expression of HOXA10 mRNA and protein was examined by qRT-PCR and western blotting in Ishikawa cells treated with different concentrations of rIL-10 for 12 h ((a) and (c)) or with 100 ng/mL rIL-10 for different times ((b) and (d)). The expression levels of STAT3, p-STAT3, and HOXA10 were examined by western blotting in Ishikawa cells treated with 4.6 μM cryptotanshinone for 24 h, followed by 100 ng/mL rIL-10 for 12 h, or in controls (e).

(a)

(b)

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(d)

(e)