Electric Pulses Can Influence Galvanotaxis of Dictyostelium discoideum

Figure 3

The waveforms of the chamber and the stimulator, the collective galvanotaxis trajectory, and the analysis of the directedness, trajectory speed, and EMR under 5 Hz pDCEF with duty cycle of 50% and 90%, and 10V/cm DCEF. (a-c) The waveforms of the chamber (blue) and the stimulator (red) for 5 Hz 50% duty cycle pDCEF, 5 Hz 90% duty cycle pDCEF, and 10 V/cm DCEF. (d-f) The galvanotactic trajectory of all 240 cells under 5 Hz 50% duty cycle pDCEF, 5 Hz 90% duty cycle pDCEF, and 10 V/cm DCEF. They all migrated towards the “net cathodes”. (g) The cells under 5 Hz 50% pDCEF was lower than 90% pDCEF and 10V/cm DCEF in directedness. ANOVA on different duty cycle: p < 0.001. For multiple comparisons, 50% versus 90%: p < 0.01; 50% versus 100% (DCEF): p < 0.001. (h) The cells under 5 Hz 90% pDCEF migrated the fastest, and 50% pDCEF the slowest. ANOVA on different duty cycle: p < 0.0001. For multiple comparisons, 50% versus 90%: p < 0.0001; 90% versus 100%: p < 0.0001; 50% versus 100% (DCEF): p < 0.0001. (i) The cells under 90% pDCEF have better EMR than that of 50%. ANOVA on different duty cycle: p < 0.0001. For multiple comparisons, 50% versus 90%: p < 0.0001. One-way ANOVA and Tukey’s test of multiple comparisons were used.

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