In Vitro Neural Differentiation of Bone Marrow Mesenchymal Stem Cells Carrying the FTH1 Reporter Gene and Detection with MRI

Figure 3

FTH1 and Flag-tag expression in MSCs. (a) Detection of the FTH1 gene in MSCs via Western blot. MSCs-FTH1 exhibited a positive protein band at 21 KDa, which was consistent with the theoretical size of the FTH1 protein. The positive band was not observed in MSCs and MSCs-LV in the control group. (b) Detection of the Flag-tag in MSCs via Western blot. A positive band near FTH1 was observed, which was of the expected molecular weight of the recombinant FTH1 (21 KDa) and Flag (1 KDa) proteins. (c) Detection of the Flag protein in MSCs using immunofluorescence. Red fluorescence was observed in MSCs-FTH1 and MSCs-LV but not in MSCs. These results confirmed that transduction with TFH1 was successful.

(a)

(b)

(c)