Intermittent Hypoxia Enhances THP-1 Monocyte Adhesion and Chemotaxis and Promotes M1 Macrophage Polarization via RAGE

Figure 3

Intermittent hypoxia promoted CCR2 expression via RAGE, which mediated chemotaxis and adhesion of THP-1 cells. Expression levels of CCR2 in THP-1 cells exposed to normoxia and intermittent hypoxia determined by qRT-PCR (a) and western blotting (b). The effects of CCR2 neutralizing antibody (10 μg/m) and CCR2 inhibitor (10 nM) on (c) the adhesion of THP-1 monocytes to HUVECs and the cell transwell migration (d). Data were represented as mean + SEM. represents significant difference compared with control group under normoxia condition; p<.05, /^##p<.001, /^###p<.001.

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