MMP-2, MMP-9, and TIMP-4 and Response to Aspirin in Diabetic and Nondiabetic Patients with Stable Coronary Artery Disease: A Pilot Study

Table 2

MMP-2, MMP-9, and TIMP-4 concentrations in aspirin good responders and aspirin-HPR patients (according to different criteria).

MMPs/TIMP-4

LTA (Amax > 20%)

value

LTA (Amax > 15%)

value

MEA

value

TXB2 > 3.1 ng/ml

value

TXB2 > 5.8 ng/ml

value

(highest quartile)

(AspiTEST > 30 AU)

(highest quartile)

HPR +

HPR −

HPR +

HPR −

HPR +

HPR −

HPR +

HPR −

HPR +

HPR −

Plasma level

MMP-2 (ng/ml)

141.8 ± 21.2

155.1 ± 6.38

0.43

153.4 ± 11.3

154.8 ± 7.0

0.09

168.6 ± 15.1

149.9 ± 6.1

0.16

161.9 ± 12.4

149.7 ± 6.5

0.08

137.6 ± 7.9

160.6 ± 7.8

0.11

MMP-9 (ng/ml)

33.7 ± 6.8

47.0 ± 3.3

0.29

49.1 ± 5.4

45.9 ± 3.6

0.17

53.4 ± 6.6

43.6 ± 3.5

0.55

50.2 ± 4.8

41.4 ± 3.8

0.14

51.5 ± 7.0

42.6 ± 3.1

0.78

TIMP-4 (pg/ml)

1796.3 ± 354.1

1614.1 ± 94.4

0.33

1757.5 ± 222.8

1593.9 ± 99.7

0.44

1502.9 ± 136.8

1676.9 ± 114.1

0.17

1379.7 ± 106.7

1726.2 ± 127.8

0.87

1466.5 ± 140.0

1630.5 ± 112.2

0.06

Supernatant of PRP after LTA induced by AA

MMP-2 (ng/ml)

141.1 ± 21.3

153.6 ± 6.0

0.70

153.3 ± 10.0

153.1 ± 6.7

0.06

166.4 ± 14.4

148.0 ± 5.9

0.90

157.1 ± 11.7

149.7 ± 6.3

0.15

134.2 ± 8.4

159.2 ± 7.3

0.13

MMP-9 (ng/ml)

33.0 ± 7.2

44.7 ± 2.67

0.12

48.3 ± 5.8

43.3 ± 2.8

0.34

43.3 ± 4.5

43.9 ± 3.1

0.14

43.9 ± 3.3

43.8 ± 3.5

0.78

42.8 ±.4.5

44.2 ± 3.0

0.62

TIMP-4 (pg/ml)

1762.6 ± 346.2

1568.9 ± 89.9

0.45

1694.0 ± 207.7

1553.0 ± 95.4

0.09

1465.3 ± 128.7

1629.6 ± 108.8

0.07

1364.5 ± 101.8

1662.4 ± 122.4

0.80

1440.9 ± 136.7

1579.4 ± 106.5

0.76

Supernatant after 5 minutes of aspirin incubation and subsequent LTA induced by AA

MMP-2 (ng/ml)

135.6 ± 19.2

146.9 ± 5.9

0.22

148.1 ± 10.9

146.0 ± 6.5

0.08

157.7 ± 12.5

142.9 ± 6.2

0.08

151.6 ± 11.8

142.8 ± 6.4

0.08

129.2 ± 7.4

152.4 ± 7.2

0.34

MMP-9 (ng/ml)

35.8 ± 6.5

44.7 ± 2.8

0.53

51.7 ± 7.8

42.8 ± 2.8

0.41

40.6 ± 3.7

45.4 ± 3.4

0.36

45.1 ± 2.8

42.6 ± 3.5

0.78

44.2 ± 4.0

43.4 ± 3.0

0.08

TIMP-4 (pg/ml)

1748.0 ± 340.3

1548.4 ± 91.4

0.07

1669.4 ± 208.9

1533.7 ± 97.7

0.76

1459.3 ± 130.8

1604.1 ± 110.7

0.73

1360.7 ± 103.2

1632.7 ± 124.8

0.67

1439.7 ± 140.6

1553.7 ± 107.8

0.16

Notes. AA: arachidonic acid; AspiTEST: arachidonic acid-induced aggregation in MEA; LTA: light transmittance aggregometry; MEA: multielectrode aggregometry; MMP-2: matrix metalloproteinase-2; MMP-9: matrix metalloproteinase-9; TIMP-4: tissue inhibitor of matrix metalloproteinase-4; TXB2: thromboxane B2; HPR: high on treatment platelet reactivity; mean ± SEM.