Research Article

Identification and Characterization of a Splicing Variant in the 5′ UTR of the Human TLR5 Gene

Figure 3

Expression of TLR5 splicing variants in different cell lines. Eleven cell lines were cultured at specific conditions. Cells were collected and 2.5 μg mRNA was used for RT-PCR; 3 μl of each cDNA was used for PCR with alternative splicing detecting primer (35 cycles, 60°C annealing temperature). GAPDH was used as control.