Research Article

Identification and Characterization of a Splicing Variant in the 5′ UTR of the Human TLR5 Gene

Figure 2

Alignment of sequences of two TLR5 splicing variants with TLR5 sequence from NCBI. Two PCR product bands of the amplification from exon IV to exon VI were collected and sequenced with automated sequencing methods by Applied Biosystems 3730xl DNA Analyzer. These two sequences were then compared to the NCBI TLR5 sequence (NCBI reference sequence: NM_003268.5) by using BioEdit software version 7.2.5. The detailed sequences are listed in Table 2.