Monoclonal Antibodies Radiolabeling with Rhenium-188 for Radioimmunotherapy

Table 2

Pros and cons between direct and indirect labeling methods.

Labeling methods	Pros	Cons
Direct	(i) Simpler approach; (ii) Less complicated antibody preparation; (iii) Fewer reaction steps; (iv) Reactions frequently conducted at room temperature; (v) Rapid procedure; (vi) More appropriate for freeze-dried kit formulation	(i) Site unspecific; (ii) Less stable radioimmunoconjugates
Indirect	(i) Site specific; (ii) More stable radioimmunoconjugates	(i) More complicated approach; (ii) More complicated antibody preparation; (iii) More reaction steps; (iv) Reactions frequently conducted at high temperature; (v) Slow procedure; (vi) Postradiolabeling purification