CMIP Promotes Proliferation and Metastasis in Human Glioma

Figure 1

CMIP promotes proliferation of human glioma cells. (a) mRNA level of CMIP was examined in human glioma cells H4, A172, and U251 by using RT-qPCR. (b) Protein level of CMIP was tested after transfection with CMIP-siRNA or negative control siRNA in A172 cells and after transfection with pIRESneo3-CMIP or pIRESneo3-Negative control in U251 cells by Western blot. (c) MTT assay and (d) cell colony formation assay were carried out in A172 cells after transfection with CMIP-siRNA or negative control siRNA and in U251 cells after transfection with pIRESneo3-CMIP or pIRESneo3-Negative control, respectively. .

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