Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit

Figure 3

Amino acid sequence alignment of Leishmania Casein Kinase I proteins. (a) The amino acid sequences of LdCK1.1 (Leishmania donovani LdBPK_351020.1, E9BRX8), TbCK1.1 (Trypanosoma brucei Tb927.5.790, Q57W24), TcCK1.1 (Trypanosoma cruzi TcCLB.508541.220, Q4DN97), TgCK1 (Toxoplasma gondii CK1, Q6QNM1), PfCK1 (Plasmodium falciparum CK1, C6S3F7), SpCK1 (Schizosaccharomyces pombe hhp1, P40235), hsCK1α (human CSNK1A1, P48729), HsCK1δ (human CSNK1D, P48730), and HsCK1ε (human CSNK1E, P49674) have been compared and the alignments were computed using the M-Coffee mode of T-Coffee. The resulting alignments were visualized using Clustalw. corresponds to amino acid residues that are invariant in all four CK1s. The dotted line marks the N-terminal domain, the black line marks the C-terminal domain, and the rest is the kinase domain. (b) The amino acid sequences of LdCK1.1 and LdCK1.2 (LdBPK_351030.1) have been compared and the alignments were computed using the M-Coffee mode of T-Coffee. The resulting alignments were visualized using Clustalw. corresponds to amino acid residues that are invariant in all four CK1s.

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