Research Article

RNA-Based Stable Isotope Probing Suggests Allobaculum spp. as Particularly Active Glucose Assimilators in a Complex Murine Microbiota Cultured In Vitro

Table 1

Summary of bacterial taxa differing between “heavy” and “medium” fractions of the 2 h and 4 h incubations with 40 mM [U13C]glucose. Shown are relative frequencies (mean ± SEM) in the “heavy” and “medium” fractions. Differences between the mean relative abundances found in these fractions were determined by one-way ANOVA. n.d. = not detected; Uc = unclassified.

Taxon2 h 13C4 h 13C
“Heavy” (%)“Medium” (%) value“Heavy” (%)“Medium” (%) value

Allobaculum0.020.56
UcLachnospiraceae0.040.15
UcPorphyromonadaceae0.030.61
UcClostridiales0.0950.29
UcRuminococcaceae0.030.2
Dorea0.030.37
Parabacteroides0.0990.17
UcDesulfovibrionaceae0.040.79
Akkermansia0.0010.36
UcFirmicutes0.170.002
UcBifidobacteriaceaen.d.0.01
UcBacteria0.080.01
Butyricicoccus0.580.09
Lactobacillus0.170.41