RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism

Figure 1

Analysis of fermentation liquor of strains Rut-C30, Δxyr1, and xyr1-rec in lignocellulosic and glucose medium. (a) SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of culture supernatants of Rut-C30, Δxyr1, and xyr1-rec. For each sample, 10 μl supernatant was loaded. (b) Protein concentration, (c) FPase activity, (d) xylanase activity, (e) biomass, and (f) protein concentration (normalized by biomass of samples) of Rut-C30 and Δxyr1 in lignocellulosic and glucose medium. For (b), (e), and (f), samples were collected after cultivation for 1, 2, 3, and 7 d. For SDS-PAGE (a) and characterization of FPase activity (c) and xylanase activity (d), samples collected 7 d after fermentation were used. All values presented in (b)–(f) are means of three independent measurements; error bars indicate standard deviations. .

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