Changes in the Expression of Biofilm-Associated Surface Proteins in Staphylococcus aureus Food-Environmental Isolates Subjected to Sublethal Concentrations of Disinfectants
Table 2
Differentially regulated proteins in biofilm samples treated with two disinfectants as determined using LC-LTQ/Orbitrap MS.
Description
PGA
Ethanol (E)
Chloramine T (C)
Differences between disinfectants
Median
QD
Median
QD
Adherence
Clumping factor A
Q6GB45
23
3.990
0.119
45
3.650
0.648
Clumping factor B
Q6G644
6
2.358
0.159
11
0.845
0.316
EC
Elastin-binding protein EbpS
Q6G983
2
0.879
0.164
8
1.259
0.440
Enolase
A7WZT2
88
0.087
83
1.131
0.203
Extracellular adherence protein Eap
D9RNP1
4
3.271
0.379
6
2.807
0.144
Fibrinogen-binding protein
P68799
2
1.334
0.109
4
2.114
0.059
Fibronectin-binding protein A
Q6G6H3
4
0.079
8
1.655
0.125
EC
Immunoglobulin-binding protein A
Q8NYT0
75
0.058
45
0.010
Immunoglobulin-binding protein sbi
Q6G6Q3
47
1.368
0.263
38
1.088
0.165
EC
Iron-regulated surface determinant protein A
A7X148
5
1.358
0.058
13
0.040
EC
Cell wall synthesis and organization
Lipoteichoic acid synthase
Q2FIS2
13
0.322
15
0.407
0.292
N-acetylmuramoyl-L-alanine amidase sle1
Q2FJH7
9
0.885
0.361
4
0.008
EC
Probable transglycosylase SceD
A7X6T9
2
2.033
0.025
4
0.050
EC
Physiological proteins
Cold shock protein CspA
Q2FH36
7
0.067
0.335
4
0.009
Large-conductance mechanosensitive channel
A7X204
6
1.527
0.065
4
1.306
0.029
Transport through membrane
Foldase protein PrsA
A7X3U8
19
1.287
0.342
17
0.066
EC
Uncharacterized proteins
Uncharacterized lipoprotein MW0073
Q8NYU0
1
1.256
0.000
7
0.852
0.072
Uncharacterized lipoprotein SAS2259
Q6G6V2
16
1.461
0.177
15
1.259
0.106
UPF0478 protein SA1560
Q7A531
22
0.102
27
1.245
0.125
EC
Virulence factors
Penicillin-binding protein 1
Q8NX37
4
1.247
0.041
2
0.323
0.009
Phospholipase C
A5IUH1
4
5.909
2.231
2
5.252
0.118
Secretory antigen SsaA-like protein
A6QEX4
2
5.578
0.106
0
Serine-aspartate repeat-containing protein C
Q6GBS6
32
2.819
0.404
12
0.054
EC
Staphylococcal secretory antigen ssaA2
Q2G2J2
22
4.088
2.288
21
0.135
EC
Virulence factor EsxA
Q5HJ91
16
2.002
0.980
17
2.261
1.384
PGA: protein group accession number; : number of peptides; QD: quartile deviation. Italic/bold numbers represent statistically significant fold changes in the indicated proteins (upregulated/downregulated) (; ; Wilcoxon signed rank test with theoretical median = 1.0). Statistically significant differences between disinfectants (; ; Mann-Whitney test followed by post hoc tests).
