In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection

Table 1

Sequences of oligonucleotide primers used for PCR amplification of biofilm-associated genes.

Gene	Nucleotide sequence of primers ().		Annealing temperature	Amplicon size (bp)	References
	Forward primer	Reverse primer
icaA (intercellular adhesion gene)	ACACTTGCTGGCGCAGTCAA	TCTGGAACCAACATCCAACA	53°C	188	[17]
icaD (intercellular adhesion gene)	ATGGTCAAGCCCAGACAGAG	AGTATTTTCAATGTTTAAAGCA	53°C	198	[17]
icaB (intercellular adhesion gene)	CCCAACGCTAAAATCATCGC	ATTGGAGTTCGGAGTGACTGC	53°C	1080	[18]
icaC (intercellular adhesion gene)	CATGAAAATATGGAGGGTGG	TCAAACTGATTTCGCCCACCG	50°C	1000	[18]
fnbA (fibronectin-binding protein A)	ATCAGCAGATGTAGCGGAAG	TTTAGTACCGCTCGTTGTCC	55°C	198	[19]
fnbB (fibronectin-binding protein B)	AAGAAGCACCGAAAACTGTG	TCTCTGCAACTGCTGTAACG	55°C	198	[19]
clfA (clumping factor A)	ATTGGCGTGGCTTCAGTGCT	CGTTTCTTCCGTAGTTGCATTTG	55°C	292	[20]
cna (collagen-binding protein)	AAAGCGTTGCCTAGTGGAGA	AGTGCCTTCCCAAACCTTTT	55°C	192	[12]
sarA (staphylococcal accessory regulatory locus)	CCCAGAAATACAATCACTGTG	AGTGCCATTAGTGCAAAACC	53°C	720	[18]
hla (alpha toxin)	CAACTGATAAAAAAGTAGGCTGGAAAGTGAT	CTGGTGAAAACCCTGAAGATAATAGAG	50°C	200	[17]