A High Diversity in Chitinolytic and Chitosanolytic Species and Enzymes and Their Oligomeric Products Exist in Soil with a History of Chitin and Chitosan Exposure

Figure 5

(a) and (b). TLC analysis of the products of chitosan (DA 35%) incubation with crude extracts of selected fungal soil isolates (numbers correspond to Table 1). Crude extracts were incubated with (samples labeled 3s, 7s, 10s, 12s, 17s, 20s, and 23s) or without chitosan (samples labeled 3c, 7c, 10c, 12c, 17c, 20c, and 23c) as a substrate. Chitosan incubated without any crude extract (co) was used as a control, and oligomers of GlcNAc (An) and GlcN (Dn) were used as standards. The DP of the standards is given on the left sides of the plates.

(a)

(b)