Kidney Injury Molecule-1 and Cardiovascular Diseases: From Basic Science to Clinical Practice

Figure 3

The effects of chloroquine (0.3 mg/kg, i.v; 3 mg/kg, i.v.) on renal I/R injury and histological micrographs of renal tissues: KIM-1 staining score. Chloroquine, in dose of 0.3 and 3 mg/kg, i.v., was injected 30 min before ischemia. Control groups, Sham + Saline, and IR + Saline received instead of drug i.v. bolus of 0.5 mL saline only (unpublished data from our laboratory projects: Professor Milica Prostran (ON175023) together with Professor Gordana Basta-Jovanovic (ON175059)). Histological micrographs of renal tissues: kidney sections taken from Sham-operated rats or rats subjected to renal I/R injury. Kidney injury molecule-1 (KIM-1) staining. Original magnification ×200. Figures were randomly chosen from the series of at least 6 experiments (a–d). (a) Sham-operated animals treated with saline only: absence of immunoreactivity for KIM-1. (b) Rats subjected to renal I/R injury, pretreated with chloroquine at 0.3 mg/kg, i.v. 30 min, before ischemia: most of proximal and some distal tubules show mild staining for KIM-1. (c) Rats subjected to renal I/R injury, pretreated with chloroquine at 3 mg/kg, i.v. 30 min, before ischemia: most of proximal and some distal tubules show moderate staining for KIM-1. (d) Rats subjected to renal I/R injury, pretreated with saline only: proximal and distal tubules show moderate to intensive positive KIM staining.

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