Research Article

Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis

Table 4

Comparison of published transgenic lines producing VLC-PUFAs and biosynthetic intermediates.

ReferencePlant speciesTissueGLASTADGLAARAETAEPADPA

This work [63 transgenic T2 line in Table 3]
(Conversion efficiencies)
A.  thaliana Seed2.3
(7.0%, D6-des)
3.9
(21.3%, D6-des)
3.1
(59.2%, C18-elo)
0.3
(8.8%, D5-des)
2.9
(42.6%, C18-elo)
0.2
(6.5%, D5-des)
0.3
(60%, C20-elo)
Abbadi et al. [23]N.  tabacum Seed29.31.81.5
L.  usitatissimum Seed16.811.41.21.00.90.8
Kinney et al. [24]G.  max Seed11.71.110.12.22.419.60.8
Cheng et al. [25]B.  carinata Seed26.95.42.25.72.520.44.0
Robert et al. [26]A.  thaliana Seed0.61.81.91.60.43.20.1
Hoffmann et al. [27]A.  thaliana Seed>0.5>0.10.80.10.90.05

Where shown, conversion efficiencies are calculated as [product]/[product + substrate] × 100. This value represents the overall percent of conversion of total substrate into total product formed.