The Omega-3 Fatty Acid Docosahexaenoic Acid Modulates Inflammatory Mediator Release in Human Alveolar Cells Exposed to Bronchoalveolar Lavage Fluid of ARDS Patients

Figure 7

Effects of ω-3/ω-6 PUFA ratios on PPARγ expression. PPARγ relative protein content in A549 cells stimulated with BALF and treated with 50 μM 1 : 2 or 1 : 7 DHA/AA ratios. Data are expressed as “a.u.” (arbitrary units) of the densitometric values, normalized on the corresponding β-actin. The value of unstimulated cells was arbitrarily set as 100. Data are presented as mean ± standard deviation of 6 independent determinations (). The image is representative of all the WB experiments. PUFA, polyunsaturated fatty acid; PPAR, peroxisome proliferator-activated receptor; BALF, bronchoalveolar lavage fluid; DHA, docosahexaenoic acid; AA, arachidonic acid; WB, western blot. BALF versus unstimulated cells. 1 : 7 DHA/AA versus BALF and unstimulated cells. 1 : 2 DHA/AA versus BALF and unstimulated cells. 1 : 2 DHA/AA versus 1 : 7 DHA/AA.