The Omega-3 Fatty Acid Docosahexaenoic Acid Modulates Inflammatory Mediator Release in Human Alveolar Cells Exposed to Bronchoalveolar Lavage Fluid of ARDS Patients

Figure 6

Effects of ω-3/ω-6 PUFA ratios on NF-κB. (a) p65 NF-κB relative protein content in the cytoplasmic fraction of A549 cells, stimulated with BALF and treated with 50 μM 1 : 2 or 1 : 7 DHA/AA ratios. Data are expressed as “a.u.” (arbitrary units) of the densitometric values, normalized on the corresponding β-actin. The value of BALF was arbitrarily set as 100. Data are presented as mean ± standard deviation of 6 independent determinations (). The image is representative of all the WB experiments. (b) NF-κB relative protein content in the nuclear fraction of A549 cells, stimulated with BALF and treated with 50 μM 1 : 2 or 1 : 7 DHA/AA ratios. Data are expressed as “a.u.” (arbitrary units) of the densitometric values, normalized on the corresponding lamin A/C. The value of BALF was arbitrarily set as 100. Data are presented as mean ± standard deviation of 6 independent determinations (). (c) IκBα relative protein content in the cytoplasmic fraction of A549 cells, stimulated with BALF and treated with 50 μM 1 : 2 or 1 : 7 DHA/AA ratios. Data are expressed as “a.u.” (arbitrary units) of the densitometric values, normalized on the corresponding β-actin. The value of BALF was arbitrarily set as 100. Data are presented as mean ± standard deviation of 6 independent determinations (). The image is representative of all the WB experiments. PUFA, polyunsaturated fatty acid; NF-κB, nuclear factor-kappa B; BALF, bronchoalveolar lavage fluid; DHA, docosahexaenoic acid; AA, arachidonic acid; WB, western blot; IκB, inhibitor of NF-κB. 1 : 2 DHA/AA versus all. 1 : 2 DHA/AA versus all. 1 : 2 DHA/AA versus all.

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