Inactivation of Src-to-Ezrin Pathway: A Possible Mechanism in the Ouabain-Mediated Inhibition of A549 Cell Migration

Figure 5

Effects of Src inhibitor, PP2, on cell migration and on the phosphorylation of ezrin and paxillin. (a) In vitro migration assay was performed twice in triplicate using a 48-well Boyden chamber with a gelatin-coated polycarbonate membrane. Serially diluted PP2 was added into the bottom chamber and cells were loaded into the upper chamber. Following incubation at 37°C for 6 h, the cells on the upper side of the membrane were removed, and the cells on the bottom of the filter membrane were stained with Diff-Quick solution. (b) The numbers of migrated cells were counted under a light microscope. The data are presented as mean ± standard deviation (; ; ). (c) Src inhibitor, PP2, was treated for indicated time and then Western blot analysis was performed as described in Materials and Methods.

(a)

(b)

(c)