HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of Picrorhiza kurroa

Table 5

Accuracy of the HPTLC methods for the estimation of cucurbitacins B and E, betulinic acid, picrosides 1 and 2, and apocynin.

% of standard spiked to the sample	Theoretical content (µg mL^−1)	Amount of drug recovered (µg mL^−1)	% of drug recovered	% RSD
Cucurbitacin B
0	300	299.66	99.88	0.51
50	450	450.66	100.14	0.34
100	600	600.67	100.11	0.25
150	750	751.33	100.17	0.07
Cucurbitacin E
0	70	71.0	101.42	1.40
50	105	105.0	100.00	0.95
100	140	141.0	100.71	0.70
150	175	175.33	100.19	0.32
Cucurbitacin D
0	250	248.58	99.43	0.17
50	350	351.15	100.3	0.58
100	500	498.21	99.64	0.36
150	650	647.24	99.57	0.5
Betulinic acid
0	34	33.83	99.50	1.03
50	51	51.5	100.98	0.97
100	68	68.96	101.42	0.65
150	85	85.43	100.50	0.99
Picroside 1
0	50	50.66	101.33	1.13
50	75	75.0	100.00	1.33
100	100	100.0	100.00	1.00
150	125	125.0	100.26	0.92
Picroside 2
0	130	131.66	101.28	0.43
50	195	196.00	100.51	0.52
100	260	261.66	100.64	0.22
150	325	324.66	99.89	0.17
Apocynin
0	50	49.33	98.66	1.17
50	75	74.33	99.11	0.77
100	100	99.0	99.00	1.01
150	125	124.66	99.73	0.46