Ouabain-Induced Cytoplasmic Vesicles and Their Role in Cell Volume Maintenance

Table 2

Effect of various treatments on intracellular water content of liver slices in the presence and absence of ouabain.

Intracellular water content (kg water/kg dry wt)^a
Incubation^b
Treatment	90 min at 1°C	Then, at 38°C for 60 min
		Control	Treatment	Ouabain	Ouabain plus treatment	()
Cytochalasin B (100 g/mL)	2.42 ± 0.33	1.48 ± 0.10	1.60 ± 0.17	1.72 ± 0.10	2.19 ± 0.11^c	8
Cytochalasin E (33 g/mL)	2.77 ± 0.07	1.75 ± 0.13	1.92 ± 0.08	1.96 ± 0.06	2.38 ± 0.13^c,d	18
Colchicine (1 mM)	2.73 ± 0.10	1.68 ± 0.11	1.61 ± 0.08	1.98 ± 0.17	2.15 ± 0.25	10
Cl^− free medium, with N as substitute	2.60 ± 0.09	1.52 ± 0.08	1.70 ± 0.08	2.00 ± 0.06	2.47 ± 0.13^c	15

 ^aIntracellular water was determined from total tissue water, measured gravimetrically, and the volume of distribution of inulin [9]. Values are the mean ± standard error of the mean.
^bSlices were incubated in a medium containing 146 mM Na⁺, 5 mM K⁺, 1 mM Mg²⁺, 1.3 mM Ca²⁺, 161 mM Cl⁻, 2 mM phosphate, 1 mM S, and Tris (10 mM pH 7.4). It was gassed with O₂. Cl-free medium contained N instead of Cl⁻. Ouabain was at 2 mM. Cytochalasins were added from stock solutions in dimethyl sulfoxide; final concentration in medium was 0.1% (v/v) DMSO.
^cSignificantly greater than value with ouabain alone, by -test; .
^dSignificantly less than value after incubation at 1°C, by -test; .