Ouabain-Induced Cytoplasmic Vesicles and Their Role in Cell Volume Maintenance

Figure 1

Liver slices after 90 min incubation at 1°C [6]. (a) Low magnification. Cells are highly swollen with volume increase. Cisternae of the endoplasmic reticulum are dilated; mitochondria are mostly in intermediate-condensed form, sometimes swollen. Cytosol and nucleoplasm are rather electroclear decreased and disorganized extracellular spaces. (b) Detail of dilated endoplasmic reticulum, detachment of ribosomes, and boundaries of cells that became uncertain with disorganized microvilli; increase and disorganization of extracellular spaces (sinusoidal, lateral, and canalicular regions). (c) Additional details: cisternae of the endoplasmic reticulum are dilated; mitochondria mostly in intermediate-condensed form, occasionally swollen; cytosol and nucleoplasm are rather electroclear (diluted). S = sinusoid; BC = bile canaliculus; N = nucleus.