Organotypic Cultures of Intervertebral Disc Cells: Responses to Growth Factors and Signaling Pathways Involved

Figure 6

Effect of growth factors on AF cells cultured in relaxed collagen gels. Bovine AF cells were cultured in relaxed collagen gels and stimulated with 10 ng/mL PDGF-BB (P), 5 ng/mL bFGF (F), and 100 ng/mL IGF-I (I). In (a) tritiated thymidine incorporation was determined after 24 hours, while in (b) cell lysates were collected after 1 hour and subjected to SDS-PAGE and Western analysis. In (a) mean values (±SEM) of three independent experiments are shown (), while in (b) one out of two similar experiments is presented.

(a)

(b)