In Vitro and In Vivo Cytogenotoxic Effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.

Table 3

Genotoxic effects of hot aqueous extract of A. satureioides (As-HAE) on erythrocytes from bone marrow of mice.

Treatments	Animals^a	PCE/NCE ± SD; (TI)^b; 24 h	MNPCE^c(); 24 h
Negative control; (saline solution)	6	1.38 ± 0.35	5.75 ± 1.77
Hot aqueous extract of A. satureioides; (As-HAE); (mg/kg bw)
100	6	0.23 ± 0.02	4.50 ± 1.54
200	6	0.22 ± 0.08	12.50 ± 1.31
500	6	0.30 ± 0.05	28.25 ± 2.35
Positive control; (cyclophosphamide)	6	1.20 ± 0.60	26.50 ± 8.02

Six mice were used per experimental group (3 males and 3 females). ^bToxicity index; ^cmicronucleated polychromatic erythrocytes. In all cases, 2000 polychromatic erythrocytes (PCE) per animal were analyzed; < 0.01, < 0.001 statistically significant difference from saline group (ANOVA Tukey test).