Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase: A UV Spectroscopic and Mass Spectrometric Study

Table 2

Mass list of the most abundant digestion products of HA after exhaustive digestion with bovine testicular hyaluronidase.

Hyaluronan oligosaccharide	Monoisotopic molecular masses
	Number of sulfate residues
	0	1	2	3
Tetrasaccharide (HA-4)	776.23	856.19	936.15	1016.10
Hexasaccharide (HA-6)	1155.34	1235.30	1315.26	1395.22
Octasaccharide (HA-8)	1534.46	1614.41	1694.37	1774.33

The table includes the masses of sulfated derivatives with up to three sulfate residues per oligosaccharide. Please note that all data were calculated by using the monoisotopic masses. Since the data will be used to explain the mass spectra (vide infra), charge compensation by protonation (not by alkali metal ions) is exclusively assumed to occur.