BioMed Research International / 2014 / Article / Fig 4

Research Article

Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase: A UV Spectroscopic and Mass Spectrometric Study

Figure 4

Dependence of the Reissig absorption (as a measure of the reducing end groups, i.e., the extent of the enzymatic digestion) on the NaCl concentration. Since the HA sample with the highest sulfate content (dss = 3) was most refractive to the hyaluronidase digestion, only this sample was investigated. All samples were digested with BTH for 20 hours at 37°C at pH 5.7 prior to measurements.

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