Analysis of Pericardial Effusion from Idiopathic Pericarditis
Patients by Two-Dimensional Gel Electrophoresis
Figure 1
(a) SDS-PAGE analysis of MicroRotofor fractions of a PE after 3 hr. of focusing at 1 W. Notice that majority of the albumin was collected in fraction 3. The shadowy darkness at an increasing pattern was due to ampholyte (pH 3–10) that was added to the protein mixture at a concentration of 2% before focusing. (b) 2D analysis of MicroRotofor fractions. Fraction 4 was the only fraction that contained detectable level of omentin-1. (c) Positions of the protein spots that were identified in fraction 4. Despite the extensive prefractionation, high abundant proteins were still dominating the gel.
