Distinct Action of Flavonoids, Myricetin and Quercetin, on Epithelial Cl− Secretion: Useful Tools as Regulators of Cl− Secretion
Figure 4
NPPB-sensitive conductance. The NPPB-sensitive conductance was measured as the difference of Isc just before and 30 min after addition of 100 μM NPPB to the apical solution. for DMSO, for quercetin, and for myricetin without forskolin ((−) FK). for DMSO, for quercetin, and for myricetin with forskolin ((+) FK). Under basal conditions ((−) FK), the values of quercetin-stimulated conductance () and myricetin-stimulated NPPB-sensitive conductance () were significantly larger than the NPPB-sensitive conductance with DMSO alone (solvent control; ). The value of Isc with DMSO alone under forskolin-stimulated conditions (DMSO marked with § in (+) FK) was significantly larger than that with DMSO alone under basal conditions (DMSO in (−) FK; ). Under forskolin-stimulated conditions ((+) FK), the value of quercetin-stimulated Isc (Quercetin in (+) FK marked with #) was slightly but significantly larger than that with DMSO alone (DMSO in (+) FK; ). Further, under forskolin-stimulated conditions ((+) FK), the value of myricetin-stimulated Isc (##) was significantly larger than that with DMSO alone (DMSO in (+) FK; ). The quercetin-stimulated NPPB-sensitive conductance under forskolin-stimulated conditions (Quercetin in (+) FK) was significantly larger than that under basal condition (Quercetin in (−) FK; ). The myricetin-stimulated NPPB-sensitive conductance under forskolin-stimulated conditions (Myricetin in (+) FK) was significantly larger than that under basal condition (Myricetin in (−) FK; ).
