Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat: Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers

Figure 6

Kidney mRNA expression of proliferation, inflammation, and angiogenesis mediators. PCNA (a), TP53 (b), mTOR (c), TGF-β ₁(d), NF-κB (e), and Mki67 (f) as proliferation status markers; CRP (g), TNF-α (h), IL-2 (i), COX-2 (j), and IL-1β (k) as inflammation status markers and VEGF (l) as angiogenesis status marker. Values are mean of CNRQ (calibrated normalized relative quantities) of the control ± SEM. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 versus control; ^#𝑃<0.05, ^##𝑃<0.01, and ^###𝑃<0.001 versus cyclosporin A. COX-2, ciclooxigenase-2; CRP, C-reactive protein; IL-1β, interleukin-1 beta; IL-2, interleukin-2; MKi67, antigen identified by monoclonal antibody Ki-67; mTOR, mammalian target of rapamycin; NF-κB, nuclear factor kappa B; PCNA, proliferating cell nuclear antigen; TGF-𝛽₁, transforming growth factor beta 1; TNF-α, tumor necrosis factor alpha; TP53, tumor protein p53; VEGF, vascular epidermal growth factor.

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