Research Article
Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method
Figure 4
Yields in various extracts from 12-week-old N. tabacum leaf strips vacuum infiltrated with 1900 μg/mL indigo carmine (). Chlorophyll produces the background absorbance observed in buffer infiltrated samples (). AWF = apoplast wash fluid; RF = rinse fluid; UHE = unwashed homogenate extract; WHE = washed homogenate extract.