Nuclear Nox4-Derived Reactive Oxygen Species in Myelodysplastic Syndromes
Figure 6
Nox4 nuclear interaction. (a) Representative images showing superimposing between DAPI (blue), Nox4 (green), and sc-35 (red) signals in MDS, MOLM-13, and THP1 cells. Scale bar: 10 μm. (b) Representative images of Western blot analysis of nuclear lysate (NL), preclearing (preclear) sample obtained, as described in methods section, before immunoprecipitation experiment with Nox4 antibody (IPNOX4): these samples were then revealed with anti-Matrin3, anti-Nox4, anti-Akt1, anti-ERK1/2, anti-sc-35, anti-Rac1, and anti-p22phox. All presented data are representative of three independent experiments.
