Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry
Figure 3
Transient expression of human FVIII in transfected cells. Cells were transfected with plasmid vectors containing wild-type full-length human F8 gene. 24 hours after transfection, cells were incubated with anti-FVIII monoclonal antibodies ab41188 and ESH8 followed by incubation with Alexa Fluor 488 labeled goat anti-mouse IgG secondary antibodies and detection by flow cytometry. Histograms depict the fluorescence intensity of 10,000 cells stained with ab41188 (red) or ESH8 (orange) antibodies. The untransfected cells are shown with dotted lines and the transfected cells with solid lines. Figures depict the ICS staining in (a) mouse fibroblasts (NIH-3T3), (b) Chinese hamster ovary (CHO), and (c) human embryonic kidney (HEK-293) cells.
