Inhibition of Carrageenan-Induced Acute Inflammation in Mice by Oral Administration of Anthocyanin Mixture from Wild Mulberry and Cyanidin-3-Glucoside
Figure 4
Effect of C3G and AF on carrageenan-induced cyclooxygenase-2 expression in peritoneal leukocytes. Groups of mice received C3G or AF (4 mg/100 g body weight) or saline by gavage in two different times: 30 min before or 1 h after cg (0.3% w/v) or saline injection into the peritoneal cavity. Peritoneal leukocytes were collected 3 h after i.p. administration of either cg or saline and whole cells were analyzed for COX-2 expression by RT-PCR and western blotting performed, as described in Section 2. (a and c) RT-PCR of COX-2, and β-actin (loading control); Bar graph shows densitometric analysis of mRNA COX-2. (b and d) Western blotting of COX-2, and β-actin (loading control) of leukocytes present in the inflammatory exudates; bar graph shows densitometric analysis of protein COX-2. The densities (in densitometry units) were normalized with those of β-actin. Results were expressed as mean ± EPM from 8 mice. # when compared with the corresponding group without cg stimulus (saline + saline). * when compared with the corresponding control group (saline + cg).
